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Question:

what chemical and molecules are needed for PCR and what is the function of each component?

i just don't know where to find this information!! I can't find it in my notes or in my teachers notes!!!

Answer:

Components required: Polymerase Enzyme - This is the actual molecule that forms a secondary DNA strand by fusing complimentary nucleotides to a phosphate backbone Polymerase Enzyme Buffer - Keeps the mix stable Magnesium: Improves replication efficiencies dNTPs: The nucleotides A,T,C G - molecules required to form the secondary strand Primers - one complimentary to the upper DNA strand and another to the lower strand - these are short oligonucleotide molecules complimentary to the start end of the sequence you wish to replicate. It acts as an anchor from which replication starts. Water - to bulk up the reaction to the required volume.

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