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Question:

What were the first experimental evidences that proved that DNA is an integral part of genetic machinery?

please explain at 1 or 2...or 3. no current experiment please like knockout mice.

Answer:

1. Griffith: injected mice with R cells mixed with dead S cells to prove bacteria can transform 2. Hershey Chase: used radioactive sulfur and phosphorous to mark proteins and DNA. injected bacteriophages in e li cell and found phosphorous entered cell, proving DNA is genetic material.
1) the Avery,Mcleod and Mccarty experiment. 2)the Hershey and Chase experiment. _ Hershey and Chase conducted their experiments on the T2 phage, a virus whose structure had recently been shown by electron microscopy.In their first set of experiments, Hershey and Chase labeled the DNA of phages with radioactive Phosphorus-32 (the element phosphorus is present in DNA but not present in any of the 20 amino acids from which proteins are made). They allowed the phages to infect E. coli.were able to observe the transfer of P32 labeled phage DNA into the cytoplasm of the bacterium.n their second set of experiments, they labeled the phages with radioactive Sulfur-35 (Sulfur is present in the amino acids cysteine and methionine, but not in DNA). Following infection of E. coli they then sheared the viral protein shells off of infected cells using a high-speed blender and separated the cells and viral coats by using a centrifuge. After separation, the radioactive S35 tracer was observed in the protein shells, but not in the infected bacteria, supporting the hypothesis that the genetic material which infects the bacteria was DNA and not protein hope it helps.:)

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