Yes, there are regulations on the use of toxic substances in agricultural plastic mulch films. These regulations vary by country but generally aim to protect the environment and human health. For instance, the European Union has the RoHS Directive (Restriction of Hazardous Substances), which restricts the use of certain hazardous substances in electrical and electronic equipment, including those used in agriculture. In the United States, the FDA oversees food contact substances, including those in agricultural films, to ensure they are safe. Compliance with these regulations is crucial to prevent contamination of soil and crops, and to ensure the safety of agricultural products for consumption.