To improve the production efficiency of diesel oil Bio oil technology
In addition to the production of biodiesel from vegetable oil by enzymatic method, has also been reported using bagasse as research results in production of high quality diesel raw material fermentation, allegedly 1 tons of bagasse energy and 1 barrels of oil equivalent (equal to 31.5 gallons per gallon per barrel, equal to 3.7853 liters). As a Canadian technology company is the achievements into productivity, plant has established 6 barrels a day of biodiesel, diesel production as raw material to bagasse, and plans to expand production plant of 25 tons per day of industrial scale. However, the use of microbial fermentation to produce diesel? What is the output rate? No specific reports.
It is a new trend to produce diesel oil by using engineering microalgae. The so-called engineering microalgae, which is constructed by genetic engineering technology, has opened up a new way for the production of diesel oil. The National Renewable Energy Laboratory (NREL) through modern biotechnology built "project microalgae", which is a "diatom Cyclotella Engineering" (Cyclotellacryptica), under laboratory conditions, the lipid content increased to more than 60% (the general lipid content under natural conditions for 5% - 20%, microalgae may also increase the production of outdoor) more than 40%. This is due to the high expression of acetyl coenzyme A carboxylase (ACC) gene in microalgae cells, and plays an important role in the control of lipid accumulation. At the present time, the suitable molecular carriers are being studied, and the ACC gene can be expressed in bacteria, yeast and plants, and the modified ACC
In order to improve the production efficiency of diesel oil, the use of enzyme immobilization technology, and the process of adding methanol in the reaction process, is more conducive to improving the production efficiency of diesel oil. The immobilized enzyme (lipase) is a kind of Candida (Candidaantaretica), which is made from the reaction column with the carrier for the production of diesel, the control temperature is 30 DEG C, the conversion rate is up to 95%. The lipase remained alive for 100 days. After several times of the reaction column, the reactants are placed in a static state and the glycerol is separated.
