Biodiesel is a renewable clean energy, its application can reduce human consumption of fossil fuels to a certain extent, the scientists found that M (single cell) in microbial lipase produced, vegetable oil and methanol to biodiesel synthesis
(1) screening of M microbial lipase production, selection and cultivation of vegetable oil based in ______ for microbial growth, cultivation of the optimum method is used ______ based sterilization method.(2) in Figure 3 is the result by using two kinds of purification methods of microbial inoculation of cultured M after inoculation culture. Inoculation method was A effect is ______, method of inoculation effect of figure B is ______.(3) if the artificial massive cloning of lipase gene, can be used _______ technology, the process is in need of special enzyme ______.
(5) the researchers proposed a target gene into M microbial cells, therefore need to select the appropriate plasmid carrier as shown in Figure 4. X-1, X-2, X-3, X-4 in four related plasmids, which Tcr said gene resistant to tetracycline resistance, Apr penicillin resistance genes, E1 and E2 expressed recognition two sites of restriction enzymes. The chemical bond restriction enzyme reaction in genetic engineering is ______. if these four plasmids were introduced into microbial M, and then M were coated on microbial bacteria containing penicillin or tetracycline in two kinds of culture medium. In the two kinds of culture medium on the growth of M are not cell types are ______.
(4) blood cell counting plate (commonly used specifications for 1mm * 1mm * 0.1mm) are divided into two kinds, one kind is each counting room including 16 middle grid, each grid consists of 25 compartments, the counting plate count when sampling statistics for the four vertex in the lattice can; the other is each including the counting chamber 25 lattice, each lattice consists of 16 compartments, the counting plate count in a lattice sampling statistics of the four corners of the central counting. Also particularly in the experiment for determination of cell number of microbial culture regular M solution, they should immediately add fixed liquid in out of sample solution. Its purpose is ______. if the liquid sample was diluted 100 times using blood cell count, as shown in Figure 2 the distribution of cell counting chamber was observed, then cultured cell density of microbial M in the solution of ______ /L.
