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Question:

Describe the ways in which bacterial machinery can be utilized to replicate mammalian genome?

Please try to explain in detail.Thanks.

Answer:

The bacteria would have to acquire mammalian DNA; the easiest way is to have it absorb the DNA into a plasmid (known as recombinant DNA); this can be induced by heat shock treatment after mixing the DNA with bacteria. This has been done with the genes for producing the enzyme, Insulin, in humans, and E. Coli bacteria (however, this insulin structure lacks certain sugars and does not function properly). Once the DNA is in a bacterial plasmid, the bacteria have ribosomes that can generate the proteins.

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