How do electrophoresis plates do?
Depending on the separation requirement, it is determined that agarose or polyacrylamide determines the concentration according to the molecular weight of the separation molecule;Melt with water and set the comb. Add the toner (or finish the gel) to the appropriate temperature
1 select the appropriate horizontal electrophoresis tank, adjust the level of the electrophoresis bath to the level. Check the voltage stabilized power supply and the positive and negative poles of the circuit;2 select the right size of the sample comb, vertical shelf at the end of electrophoresis gel mold, so that the bottom of the sample comb from the bottom of the gel electrophoresis distance of 1.0mm;3 according to the separation of DNA, preparation of the corresponding concentration of agarose, 100 degrees water heated to agarose melt evenly;4 Straw take a small amount of agarose gel electrophoresis gel solution will die around sealed to prevent water penetration occurred in agarose gel plate. Agarose gel to be cooled to about 50 DEG C, 6 L nucleic acid dye, adding shake gently into the gel mold, agarose gel thickness in 3 ~ 5mm. pour glue to avoid have a bubble, if the bubble can be sucked Straw carefully;After the 5 agarose gel is solidified, place 20min at room temperature. Carefully pull out the sample comb and the baffle at the ends of the electrophoresis gel to keep the sample hole intact;6, electrophoresis gel mold into the electrophoresis slot, add electrophoresis buffer, so that electrophoresis buffer surface higher than the agarose gel surface 1 ~ 2mm., such as point sample hole has bubbles, carefully sucked with straw, so as not to affect the sample;
10 L DNA and 2 l sample volume bromophenol blue indicator sample buffer. The mixed sample buffer can not only improve the density of the sample, the sample is evenly sink to the sample hole, also can make samples with color, easy to sample and estimate and judge the position of electrophoresis electrophoresis time;8 use micro pipette to add sample carefully to sample hole, record sample order;9, cover the electrophoresis tank, turn on the power switch, the maximum voltage is not more than 5V/cm (100 ~ 150V constant voltage electrophoresis), so that DNA moves from the negative electrode to the positive electrode;Depending on the specific requirements of the 10 time with the electrophoresis experiment. Electrophoresis generally takes 1 to 3 hours. After electrophoresis power off, wearing disposable plastic gloves as far as possible to remove gel electrophoresis buffer with all stem, observed in the transmitted UV light of 254nm wavelength.
