I have carried out a quantitative test for reducing sugars. 6 concentrations, benedicts added to each solution, heatedThey are then tested for % transmissionAs the concentration decreases the% transmission increase, but I can not explain why! Help, please!?!I also need to find out how this could be used in industry
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The measurement of % Transmission is to determine how much light of a specific wavelength can still pass through a solution without being absorbed by the sample. When you use Benedict's reagent to test solutions of reducing sugars at different concentrations, the solutions with more sugar will produce more color and thus, will also absorb more of the light that tries to pass through in the photometer. So, as the concentration of sugar is reduced, so too is the amount of colored product. As a result the %transmission will go up a sthe sugar concentration goes down. In industrial situations this test could be used to measure sugar production or sugar use (and thus reduction) by yeast in fermentation.
