What is the difference between voltage clamp and patch clamp?
Voltage clamp technology is to inject a certain amount of current into the cell to counteract the ion flow produced by the opening of the ion channel, and then fix the cell membrane potential at a certain value.
Since the magnitude of the injection current is the same as that of the ion stream, it can reflect the magnitude and direction of the ion flow. Patch clamp is clamped diaphragm, refers to the use of sophisticated processed microelectrodes and the cell membrane in close contact, make the tip of the cell membrane separation and other cell membrane in the electrical, which greatly reduces the background noise, so that the current single channel to detect the weak.
The voltage of the film is clamped to a certain value by the voltage clamp technique, and the single channel current can be recorded. From this point of view, patch clamp technique is a special voltage clamp technique. With the development of patch clamp technology, it has not only been confined to the concept of diaphragm, but also not only voltage clamp technique, but also current clamp technique.