An important property of the catalyst is that the reaction equilibrium is not changed while increasing the forward reaction rate and the reverse reaction rate. However, because the enzyme for the specificity of the substrate, is not almost every reaction by the enzyme are one way to do it.
Enzymes, refers to the biocatalytic function of the polymer material, in the enzyme catalytic reaction system, the reactant molecules known as the substrate, the substrate catalyzed by the enzyme into another molecule. Similar to other non-biocatalysts, the enzyme changes the reaction rate by adjusting the Gibbs free energy of the chemical reaction, and most of the enzyme can increase the rate of its catalytic reaction by a million times; in fact, the enzyme is provided with another The activation energy requires a lower route so that more reactive particles produce more effective collisions to produce more kinetic energy. According to the first law of thermodynamics, the kinetic energy obtained by the collision can accelerate the reaction rate by transformation. The enzyme as a catalyst itself is not consumed in the reaction process nor does it affect the chemical equilibrium of the reaction.
A brief description of restriction endonucleases and DNA ligases: Restriction endonucleases, and DNA ligases, can be said to have nothing to do with energy calculations. In particular, the actual effect of these two enzymes is not to switch between A (DNA) and B (disconnected DNA) states. Restriction endonucleases do not need to open base pairing in addition to the phosphates, whereas the DNA ligase itself is responsible for linking 5'-phosphate and 3'-hydroxy. Which use the energy supply substances, coenzyme are not the same, can not be used as the same reaction is positive and negative process considerations.
Only answer lz, the last question and. The reason is that both the restriction endonuclease and the ligase are required for certain reaction conditions and the reaction substrate. And the reaction conditions are different, so that the final results are different, of course, the enzyme is relatively speaking is not so high, perhaps the destruction is always stronger than the building it. The ligase is the need for a specific substrate, three distilled water, temperature, time (long time do not forget the formula) to control, it may have a certain proportion of connection. So this is two questions.
In biochemical reactions, most appear to be positive / inverse reaction combinations, in fact there are differences in peripheral product / energy offerings. In addition to the molecules of interest, there are many other small molecules involved in the reaction, and these small molecules in the forward or reverse reaction in the transformation is not completely mutually negative. Even for the same reaction, since the corresponding substrate may be removed in the subsequent step and the pulling balance occurs, it is also possible that the forward or reverse direction can occur. Decisive factors usually come from the surrounding other enzymes, coenzymes, small molecules concentration.
