What are the methods of calibration, external standard and internal standard in instrumental analysis?Standard plus standard method...... Thank you
External standard method: it is to use the pure substance that wants to measure the component to make the standard curve to seek the method of the unknown component
Standard: a series of known amounts of analyte were added to several aliquots of the samples, prepared concentration (cx+0), (cx+c1), (cx+c2), (cx+c3)...... A standard series that has the same matrix as the sampleYou say, plus standard method, I think that is the standard adding method, that is, after adding the mark, through the instrument to measure the above series of response values S0, S1, S2, S3, S4...... The response signal was plotted with S at the concentration of C, and then the line extrapolation and the concentration axis intersect at one point. The concentration of the analyte to be measured was cx.
The internal standard method, internal standard method is a kind of calibration method for indirect or relative. In the analysis of determination of a component content, effect of adding an internal standard substance to calibrate and eliminate the fluctuation of operating conditions for the results of the analysis, in order to improve the accuracy of analysis result.
