The chromatin is stained with alkaline dyes, where acetic acid magenta or gentian violet dye can be used. Although these two pigments are called alkaline, why is the pH of the solution acidic?
This type of dye ionization after the dye ion with a positive, can be combined with acidic substances into the salt. Microbial laboratory commonly used alkaline dyes are Meilan, methyl violet, ammonium oxalate crystal violet, acetic acid magenta, alkaline complex red, Red, malachite green and fan red, etc., in general, the bacteria easily stained with alkaline dyes.
Alkaline dyes are those which can dissociate cationic dyes in aqueous solution and are classified as cationic dyes, also known as salt-based dyes, most of which are organic bases and are formed with inorganic acids Salt (chloride) a small number of other salts. Dye in aqueous solution dissociation, because the pigment group with a positive charge, which is a cationic dye.
