i have already watched a video about how procedure is done, but i need a list of required materials , with clear concentrations and stepsthanks for your help in advance gt;gt;
First, either you have normal urine with 0.2-1.0% glucose or you have diabetic urine. If normal urine, use without diluting. If diabetic, pipet 10 mL into a 100-mL volumetric flask and make up to 100 mL with distilled water. Next prepare 1 L of Benedicts's quantitative reagent. The ingredients are: CuSO4*5H2O 18g Sodium or potassium citrate 200g (in my reference, there is so much that molecular weight seems not to matter) Potassium thiocyanate 125g Sodium carbonate anhydrous 100g Potassium ferrocyanide, 5% solution, 5 mL Distilled water to 1 L Procedure: Put all ingredients except CuSO4*5H2O into 800 mL H2O and heat to dissolve. Filter the solution. Weigh the CuSO4*5H2O very accurately on an analytical balance. Dissolve it in 100 mL water. Add that solution slowly with stirring to the other. Finally, add the 5 mL of 5% K4Fe(CN)6 solution. Cool the resulting solution and make up exactly to 1 L. In the titration, exactly 50 mg glucose reduces 25 mL of this reagent. For the titration: Measure out 25 mL of the above reagent into a 250-mL Erlenmeyer flask. Add 5-10g anhydrous sodium carbonate, add a boiling chip, and bring to a boil. Put the urine or diluted urine into a 50-mL buret. Add the urine/dilute to the reagent 0.5 mL at a time. At first, there is a milk white precipitate with a blue color. As the blue color disappears, titrate more slowly. The end point is white with a grayish-green tinge, depending on how yellow was the urine. It is important to keep the solution at a low boil throughout the titration.
